What is Ficoll method?

Ficoll (GE trademark) is high-mass polysaccharide - part of Ficoll-Paque used to separate blood to its components http://bit.ly/agqOK

Ficoll-Paque layers from top to bottom: plasma, mononuclear cells, Ficoll-Paque, erythrocytes and granulocytes http://bit.ly/GrctD

Buffy coat is a fraction of anticoagulated blood sample after density gradient centrifugation - contains WBC, platelets http://bit.ly/TvgvP

Ficoll-Paque layering is a very slow process, devices that speed the most time-consuming step (overlay) were developed http://bit.ly/agqOK

Example: Clinical efficacy and immune regulation with peanut oral immunotherapy. Jones SM et al. J Allergy Clin Immunol. 2009 Jul 2.

Ficoll-Paque layers from Basic cell culture protocols By Cheryl D. Helgason, Cindy L. Miller:

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