Some basic science methods used in allergy and immunology research

Example: Clinical efficacy and immune regulation with peanut oral immunotherapy. Jones SM et al. J Allergy Clin Immunol. 2009 Jul 2.

PBST = Phosphate Buffered Saline - Tween 20 (Polysorbate 20) is wash solution for ELISA http://bit.ly/1aLbmz and http://bit.ly/19yp8e

Bicinchoninic acid assay (BCA assay or Smith assay) is used for determining the total level of protein in a solution http://bit.ly/2J768b

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a buffering agent widely used in cell culture http://bit.ly/47H5r2

Flow cytometry: PerCP is Peridinin chlorphyll protein http://bit.ly/UsrCI

Flow cytometry: Allophycocyanin is red despite its name - Greek: (allos) "other", (phykos) alga”, (kyanos) "blue" http://bit.ly/fjZ96

RPMI (Roswell Park Memorial Institute medium) is a medium used in cell cultures. http://bit.ly/QCGDP

Entrez Gene is a searchable database of genes by NCBI | NLM | NIH http://bit.ly/jP0r


Flow cytometry from Color atlas of immunology By Gerd-Rüdiger Burmester, Antonio Pezzutto, Timo Ulrichs, Alexandra Aicher: