Some basic science methods used in allergy and immunology research

Example: Clinical efficacy and immune regulation with peanut oral immunotherapy. Jones SM et al. J Allergy Clin Immunol. 2009 Jul 2.

PBST = Phosphate Buffered Saline - Tween 20 (Polysorbate 20) is wash solution for ELISA and

Bicinchoninic acid assay (BCA assay or Smith assay) is used for determining the total level of protein in a solution

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a buffering agent widely used in cell culture

Flow cytometry: PerCP is Peridinin chlorphyll protein

Flow cytometry: Allophycocyanin is red despite its name - Greek: (allos) "other", (phykos) alga”, (kyanos) "blue"

RPMI (Roswell Park Memorial Institute medium) is a medium used in cell cultures.

Entrez Gene is a searchable database of genes by NCBI | NLM | NIH

Flow cytometry from Color atlas of immunology By Gerd-RĂ¼diger Burmester, Antonio Pezzutto, Timo Ulrichs, Alexandra Aicher:

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