The basophil activation test (BAT) is a widely validated and reliable tool especially for the diagnosis of hymenoptera venom allergy.
Basophil granulocyte. Image source: Wikipedia.
Basophil activation test should be performed as early as possible after taking the blood sample, preferably within 4 h.
In contrast to the skin test, BAT can be performed in patients undergoing treatment with antihistamines.
BAT should be performed only at validated laboratories.
Bigger granules (1.2 µm)
The basophil activation test in the diagnosis of allergy: technical issues and critical factors. Sturm GJ, Kranzelbinder B, Sturm EM, Heinemann A, Groselj-Strele A, Aberer W. Allergy. 2009 Feb 20.
Mast Cells and Basophils