Basophil activation test in venom allergy should be performed within 4 hours of taking the blood sample

The basophil activation test (BAT) is a widely validated and reliable tool especially for the diagnosis of hymenoptera venom allergy.


Basophil granulocyte. Image source: Wikipedia.

Basophil activation test should be performed as early as possible after taking the blood sample, preferably within 4 h.

In contrast to the skin test, BAT can be performed in patients undergoing treatment with antihistamines.

BAT should be performed only at validated laboratories.

Mnemonic:

B
Basophils
Blood circulation
Bigger granules (1.2 µm)
Bilobar nucleus

References:
The basophil activation test in the diagnosis of allergy: technical issues and critical factors. Sturm GJ, Kranzelbinder B, Sturm EM, Heinemann A, Groselj-Strele A, Aberer W. Allergy. 2009 Feb 20.
Mast Cells and Basophils